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Global Journal of Reproductive Medicine   Juniper Publishers


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Sperm-Oviduct Interaction Factors that Compromise Fertility of Frozen Stallion Semen

Global Journal of Reproductive Medicine   Juniper Publishers Abstract The cryopreservation procedure changes the integrity and functionality of sperm, affecting their longevity and the ability to fertilize the oocyte. Moreover, some of those changes could be alter the physiologic interactions between the sperm and the oviductal epithelial cells prior to fertilization, placing further limitations and challenges on frozen-thawed stallion sperm. For fertilization to occur, a sperm subpopulation should be survive in uterine environment and be transported to oviduct, the tubular epithelium that regulate the sperm capacitation process in tuning with the ovulation and later oocyte fecundation. Factors affecting sperm-oviduct interactions in equine and findings in other mammals suggest that this interaction is highly conserved. However, there are differences between species and this interaction may assist to explain the idiopathic infertility associated with t...
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Research Article Intrauterine Insemination with Fresh Versus Cryopreserved Spermatazoa in Unexplained Infertility

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Global Journal of Reproductive Medicine    Juniper Publishers Authored by: Sheena Rippentrop* Introduction Infertility affects approximately 14% of couples in the general population and is defined as failure to conceive after 12 months of regular unprotected intercourse. Unexplained infertility accounts for up to 15-30% of the diagnoses in couples [1,2]. The diagnosis of unexplained infertility is one of exclusion and can only be made after investigating the common causes of infertility using standard testing including evaluation for male and female infertility [1]. This evaluation typically involves assessment of ovulation, ovarian reserve, fallopian tube patency, uterine and cervical factors, along with semen analysis. In couples with infertility, previous studies have shown that ovulation induction with intrauterine insemination (IUI) provides a less invasive and more cost-effective treatment option when compared to in-vitro fertilization in couples with ...
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NK-Cell Count and its Function in Producing Interferon Gamma Associated with the Cervical Cancer Natural History-Juniper publishers

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Global Journal of Reproductive Medicine    Juniper Publishers Authored by: Tofan Widya Utami*  Abstract Objective:  Natural killer cell (NK-cell) has an important role in tumor immune surveillance. We investigate the NK-cell count and its function in producing IFN-γ on normal cervix, subclinical high risk-HPV (hr-HPV) infection, precancerous lesion, and cervical cancer in order to understand the natural history of uterine cervical cancer. Methods:  This is a descriptive comparative numerical study with more than two unpaired group with total subjects of 40 females who met the inclusion and exclusion criteria. Samples were gathered from cervical tissue using cytobrush and were processed in the laboratory to calculate NK-cell count and NK-cell expression of IFN-γ by phytahemagglutinin (PHA) stimulation using flowcytometry. Data was analyzed using Kruskal Wallis and post hoc analysis was done to determine the difference between groups. Results:...
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